Kocaeli Med J. 2025; 14(2): 135-141 | DOI: 10.5505/ktd.2025.47897

Identification of Vaginitis-Causing Microorganisms Isolated From Vaginal Discharge Cultures and Evaluation of Their Antimicrobial Susceptibility Profiles

Erkan Sanmak¹, Zeynep Ayaydin², Ömer Tammo^3
1Mardin Training and Research Hospital, Medical Microbiology Laboratory, Mardin, Türkiye
²Mardin Artuklu University, Faculty of Medicine, Department of Medical Microbiology, Mardin, Türkiye
³Harran University, Faculty of Medicine, Department of Obstetrics and Gynecology, Þanlýurfa, Türkiye

INTRODUCTION: Vaginal infections are often associated with disruption of the normal vaginal microbiota, with bacterial pathogens being the most common causative agents. This study aimed to identify the microorganisms isolated from vaginal discharge samples of patients presenting with vaginal complaints and to evaluate their antimicrobial susceptibility profiles.
METHODS: This retrospective study included 266 patients who presented to the Obstetrics and Gynecology outpatient clinics or inpatient services of XXX Training and Research Hospital with complaints of vaginal discharge and had positive vaginal culture results. Microorganism identification was performed using Gram staining, conventional biochemical methods, and the VITEK-2 system. Antimicrobial susceptibility testing was conducted according to standard laboratory protocols.
RESULTS: Among the isolated microorganisms, 49.3% were Gram-positive bacteria, 48.1% were Gram-negative bacteria, and 2.6% were identified as Candida species. The most frequently isolated pathogens were Escherichia coli (E. coli) (35.7%), coagulase-negative staphylococci (19.9%), Streptococcus agalactiae (14.6%), Staphylococcus aureus (9.3%), and Klebsiella spp. (9.3%). E. coli showed the highest susceptibility to colistin, meropenem, and imipenem, while Gram-positive isolates exhibited the greatest sensitivity to linezolid, tigecycline, and vancomycin.
DISCUSSION AND CONCLUSION: E. coli was identified as the most common pathogen associated with vaginal discharge. Understanding the antimicrobial susceptibility profiles of these isolates is essential for guiding appropriate treatment strategies and preventing recurrent infections and potential complications.

Keywords: escherichia coli, antimicrobial susceptibility, microbial identification, vaginal discharge, vaginitis

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Vajinal Akýntý Kültürlerinden Ýzole Edilen Vajinit Etkeni Mikroorganizmalarýn Tanýmlanmasý ve Antimikrobiyal Duyarlýlýk Profillerinin Deðerlendirilmesi

Erkan Sanmak¹, Zeynep Ayaydin², Ömer Tammo^3
1Mardin Eðitim ve Araþtýrma Hastanesi, Týbbi Mikrobiyoloji Laboratuvarý, Mardin, Türkiye
²Mardin Artuklu Üniversitesi, Týp Fakültesi, Týbbi Mikrobiyoloji Ana Bilim Dalý, Mardin, Türkiye
³Harran Üniversitesi, Týp Fakültesi, Kadýn Hastalýklarý ve Doðum Bölümü, Þanlýurfa, Türkiye

GÝRÝÞ ve AMAÇ: Vajinal enfeksiyonlar, vajinal mikrobiyal dengenin bozulmasýyla iliþkilidir ve sýklýkla bakteriyel etkenler rol oynamaktadýr. Bu çalýþmada, vajinal akýntý þikayetiyle baþvuran hastalardan alýnan vajinal sürüntü örneklerinde izole edilen mikroorganizmalarýn belirlenmesi ve bunlarýn antimikrobiyal duyarlýlýk profillerinin deðerlendirilmesi amaçlanmýþtýr.
YÖNTEM ve GEREÇLER: Retrospektif olarak planlanan çalýþmaya, XXX Eðitim ve Araþtýrma Hastanesi Kadýn Hastalýklarý ve Doðum birimlerine vajinal akýntý yakýnmasýyla baþvuran ve kültürlerinde mikroorganizma üremesi saptanan 266 hasta dahil edilmiþtir. Tanýmlayýcý analizlerde Gram boyama, konvansiyonel biyokimyasal yöntemler ve VITEK-2 sistemi kullanýlmýþ; antibiyotik duyarlýlýðý ise standart protokollerle deðerlendirilmiþtir.
BULGULAR: Ýzole edilen mikroorganizmalarýn %49,3’ü Gram pozitif, %48,1’i Gram negatif bakterilerden, %2,6’sý ise Candida türlerinden oluþmuþtur. En sýk tespit edilen etkenler Escherichia coli (E.Coli) (%35,7), koagülaz negatif stafilokoklar (%19,9), Streptococcus agalactiae (%14,6), Staphylococcus aureus (%9,3) ve Klebsiella spp. (%9,3) olmuþtur. E. coli colistin, meropenem ve imipeneme; Gram pozitif bakteriler ise linezolid, tigecycline ve vankomisine yüksek duyarlýlýk göstermiþtir.
TARTIÞMA ve SONUÇ: Vajinal akýntýya en sýk neden olan etkenin E. coli olduðu saptanmýþtýr. Ýzolatlarýn antimikrobiyal duyarlýlýk profillerinin bilinmesi, etkili tedavi planlamasý ve komplikasyonlarýn önlenmesi açýsýndan klinik önem taþýmaktadýr.

Anahtar Kelimeler: escherichia coli, antimikrobiyal duyarlýlýk, mikrobiyal tanýmlama, vajinal akýntý, vajinit

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