Kocaeli Med J. 2019; 8(1): 125-129 | DOI: 10.5505/ktd.2019.19327

Effects of Zoledronic Acid in BCPAP Thyroid Papillary Carcinoma Cell Line

ILGIN YILDIRIM SIMSIR¹, Gökçen ÜNAL KOCABAÞ¹, Çýðýr BIRAY AVCI², Füsun SAYGILI^1
1Ege University Medical Faculty, Division of Endocrinology and Metabolism Disorders, Izmir
²Ege University Medical Faculty, Department of Medical Biology, Izmir

INTRODUCTION: Thyroid cancer is the most common endocrine neoplasm with an incidence of 3.7% in Turkey. Mutations and re-arrangements in genes encoding proteins of the the mitogen-activated protein kinase (MAPK) pathway for the development and progression of differentiated thyroid cancer, gain more and more importance. Bisphosphonates decrease tumor cell proliferation, reduce cell viability, stimulate apopitosis in tumor cell, inhibit cell adhesion and angiogenesis, and decrease metastatic potential in vitro.
In our study, the effects of zoledronic acid monohydrate on cytotoxicity and apoptosis in thyroid papillary carcinoma cell line were investigated.

METHODS: Zoledronic acid monohydrate with increasing doses of 10 to 100 μM was used on a papillary thyroid carcinoma cell line of human origin named BCPAP.
RESULTS: IC50 value with zoledronic acid monohydrate was found in first experiment at 48th hour to be 55 μM, but 2nd, 3rd and 4th experiments were failed. Therefore, apoptosis assays were not to be established.
DISCUSSION AND CONCLUSION: Zoledronic acid was found to be ineffective in the thyroid papillary carcinoma cell line at the dose range we were working with.

Keywords: Papillary thyroid carcinoma, BCPAP cell line, zoledronic acid

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Zoledronik Asidin BCPAP Tiroid Papiller Karsinomu Hücre Hattýnda Etkileri

ILGIN YILDIRIM SIMSIR¹, Gökçen ÜNAL KOCABAÞ¹, Çýðýr BIRAY AVCI², Füsun SAYGILI^1
1Ege Üniversitesi Týp Fakültesi, Endokrinoloji ve Metabolizma Hastalýklarý BD, Ýzmir
²Ege Üniversitesi Týp Fakültesi, Týbbi Biyoloji AD, Ýzmir

GÝRÝÞ ve AMAÇ: Türkiye’de sýklýðý %3.7 olarak tanýmlanan tiroid kanserleri en sýk görülen endokrin neoplazilerdir. Mitojen aktive protein kinaz (MAPK) yolaðýndaki proteinleri kodlayan genlerdeki mutasyon ve yeniden düzenlemeler, diferansiye tiroid kanseri geliþimi ve ilerlemesi için giderek daha fazla önem kazanmaktadýrlar. Bifosfonatlar invitro olarak; tümör hücre proliferasyonunu azaltýr, hücre viabilitesini azaltýr, tümör hücre apopitosisini uyarýr, hücre adezyonu inhibisyonu yapar, anjiyogenezde inhibisyon ve metastatik potansiyelde azalma yaparlar.
Çalýþmamýzda zoledronik asit monohidratýn tiroid papiller karsinomu hücre hattýnda sitotoksisite ve apoptozis üzerine etkileri araþtýrýlmýþtýr.

YÖNTEM ve GEREÇLER: Ýnsan kökenli bir papiller tiroid karsinomu hücre hattýnda (BCPAP) bifosfonat olarak zoledronik asid monohidratýn 10-100 µM þeklinde artan dozlarý kullanýldý.
BULGULAR: BCPAP hücre hattýnda zoledronik asid monohidratýn IC50 deðeri 1. deneyde 48. saatte 55 µM olarak saptandý. Ancak 2, 3 ve 4. deneylerde sonuç alýnamadý. Bu nedenle apopitozis deneyleri kurulmadý.
TARTIÞMA ve SONUÇ: Zoledronik asit, çalýþtýðýmýz doz aralýðýnda tiroid papiller karsinomu hücre hattýnda etkisiz bulunmuþtur.

Anahtar Kelimeler: Papiller tiroid karsinomu, BCPAP hücre hattý, zoledronik asit

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